7SIT

Crystal structure of Voltage gated potassium ion channel, Kv 1.2 chimera-3m

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	90
Detector technology	PIXEL
Collection date	2020-03-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	P 4 21 2
Unit cell lengths	129.587, 129.587, 278.482
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.159 - 3.320
R-factor	0.2529
Rwork	0.251
R-free	0.28930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r9r
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.160	3.510
High resolution limit [Å]	3.320	3.320
Number of reflections	35720	4588
<I/σ(I)>	7.2
Completeness [%]	99.9
Redundancy	14.9
CC(1/2)	0.990	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	50 mM Tris, pH 8.5, 28-32% PEG400, CHAPS