7SEP
Cryo-EM Structure of the RT component of the HIV-1 Pol Polyprotein
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.8 |
| Number of Particle | 27555 |
| Software | PHENIX,RosettaEM,cryoSPARC,Warp,Coot,UCSF Chimera |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
