7SCF
M. tb EgtD in complex with HD2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-07-31 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.102, 71.570, 145.095 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.950 - 2.670 |
R-factor | 0.2193 |
Rwork | 0.213 |
R-free | 0.28010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uy5 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.950 | 2.670 |
High resolution limit [Å] | 2.670 | 2.670 |
Rmerge | 0.100 | 0.450 |
Number of reflections | 21089 | 2068 |
<I/σ(I)> | 10.89 | |
Completeness [%] | 99.8 | |
Redundancy | 1 | |
CC(1/2) | 0.960 | 0.440 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.2 M potassium phosphate dibasic and 20 % w/v polyethylene glycol 3,350 |