7S95
Room-temperature Human Hsp90a-NTD bound to adenine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 278 |
| Detector technology | PIXEL |
| Collection date | 2020-09-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.943, 89.701, 101.286 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.660 - 1.710 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yer |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.177 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19RC5_4047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.660 | 1.770 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.059 | 0.818 |
| Number of reflections | 31864 | 3104 |
| <I/σ(I)> | 9.24 | |
| Completeness [%] | 93.4 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.995 | 0.556 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.8 M Malic acid, pH 7 |
