7S8Y
Cryogenic apo Human Hsp90a-NTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 65.146, 88.394, 99.546 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.200 - 1.590 |
R-factor | 0.1659 |
Rwork | 0.165 |
R-free | 0.19130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yer |
RMSD bond length | 0.010 |
RMSD bond angle | 1.049 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19rc5_4047) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.200 | 1.650 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmerge | 0.093 | 0.678 |
Number of reflections | 38596 | 3808 |
<I/σ(I)> | 6.77 | |
Completeness [%] | 99.0 | |
Redundancy | 4.4 | |
CC(1/2) | 0.996 | 0.567 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM HEPES, pH 7 and 1.6 M sodium citrate dihydrate |