7S8H

Structure of Lassa virus glycoprotein bound to Fab 18.5C and Fab 36.1F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033190
Spacegroup name	P 3 2 1
Unit cell lengths	163.058, 163.058, 173.548
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.180 - 2.700
Rwork	0.217
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p91
RMSD bond length	0.005
RMSD bond angle	0.764
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.180	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	73503	7267
<I/σ(I)>	9.53
Completeness [%]	99.9
Redundancy	10
CC(1/2)	0.996	0.260

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	0.1 M Tris, pH 8.0, 1.25 M LiCl and 15% PEG 8k