7S80
Crystal structure of iAChSnFR Acetylcholine Sensor precursor binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.536, 60.816, 58.657 |
Unit cell angles | 90.00, 100.52, 90.00 |
Refinement procedure
Resolution | 33.900 - 1.400 |
R-factor | 0.1705 |
Rwork | 0.170 |
R-free | 0.19530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7s7t |
RMSD bond length | 0.004 |
RMSD bond angle | 0.746 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.900 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.061 | 1.209 |
Rmeas | 0.067 | 1.312 |
Rpim | 0.026 | 0.504 |
Number of reflections | 55565 | 5503 |
<I/σ(I)> | 12.83 | 1.23 |
Completeness [%] | 98.1 | 97.79 |
Redundancy | 6.7 | 6.6 |
CC(1/2) | 0.998 | 0.776 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 296 | 25% PEG 1,500 0.1M MIB, pH 5.0 |