7S7K
Crystal structure of the EphB2 extracellular domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.843, 111.142, 156.877 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.400 - 3.150 |
R-factor | 0.2153 |
Rwork | 0.214 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fl7 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.400 | 48.400 | 3.320 |
High resolution limit [Å] | 3.145 | 9.950 | 3.145 |
Rmerge | 0.014 | 0.792 | |
Rmeas | 0.057 | 0.018 | 1.040 |
Rpim | 0.030 | 0.010 | 0.544 |
Total number of observations | 73799 | ||
Number of reflections | 22540 | 746 | 3293 |
<I/σ(I)> | 18 | 42.4 | 1 |
Completeness [%] | 98.0 | 90.9 | 99.3 |
Redundancy | 3.3 | 3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.1 M Na Succinate, 0.1M Na Acetate pH 4.8, 5% (v/v) MPD, and 3% 1, 6-Hexanediol |