7S5S
CTX-M-15 WT in complex with BLIP WT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2019-06-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 47.480, 81.860, 129.120 |
| Unit cell angles | 90.00, 95.94, 90.00 |
Refinement procedure
| Resolution | 33.490 - 1.400 |
| R-factor | 0.1516 |
| Rwork | 0.151 |
| R-free | 0.16630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4HBT and 3GMU |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.947 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.490 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.074 | 0.057 |
| Number of reflections | 94955 | 511 |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 98.4 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% w/v PEG3350, 0.2 M NaF, 0.1 M Bis-Tris-propane pH 6.5 |
