7S5S
CTX-M-15 WT in complex with BLIP WT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2019-06-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 47.480, 81.860, 129.120 |
Unit cell angles | 90.00, 95.94, 90.00 |
Refinement procedure
Resolution | 33.490 - 1.400 |
R-factor | 0.1516 |
Rwork | 0.151 |
R-free | 0.16630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4HBT and 3GMU |
RMSD bond length | 0.006 |
RMSD bond angle | 0.947 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.490 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.074 | 0.057 |
Number of reflections | 94955 | 511 |
<I/σ(I)> | 14.8 | |
Completeness [%] | 98.4 | |
Redundancy | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% w/v PEG3350, 0.2 M NaF, 0.1 M Bis-Tris-propane pH 6.5 |