7S5R
Crystal structure of SARS-CoV-2 receptor binding domain in complex with neutralizing antibodies CV07-287 and COVA1-16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.632, 121.480, 136.938 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.530 - 2.450 |
R-factor | 0.2087 |
Rwork | 0.207 |
R-free | 0.23750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7jmw |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.450 | 6.650 | 2.450 |
Rmerge | 0.115 | 0.046 | 1.169 |
Rmeas | 0.119 | 0.048 | 1.270 |
Rpim | 0.033 | 0.013 | 0.480 |
Total number of observations | 694114 | ||
Number of reflections | 55625 | 3004 | 2743 |
<I/σ(I)> | 5.2 | ||
Completeness [%] | 99.9 | 99.5 | 99.2 |
Redundancy | 12.5 | 12.2 | 6.6 |
CC(1/2) | 0.999 | 0.496 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293.15 | 1.6 M ammonium sulfate and 0.1 M bicine pH 9.0 |