7S3G
Structure of cofactor pyridoxal 5-phosphate bound human ornithine decarboxylase in complex with citrate at the catalytic center
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.540, 86.470, 154.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.250 - 1.660 |
| R-factor | 0.1798 |
| Rwork | 0.178 |
| R-free | 0.19750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oo0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.315 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.250 | 1.680 |
| High resolution limit [Å] | 1.640 | 1.660 |
| Number of reflections | 122761 | 3597 |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 8.2 | |
| CC(1/2) | 0.990 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 100 mM tri-sodium citrate pH 5.0 and 30% polyethylene glycol monomethyl ether 550 |
