7S3C

Crystal structure of intact U2AF65 RRM-region bound to AdML-A5 oligonucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	43.549, 55.771, 76.414
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.840 - 1.510
R-factor	0.1607
Rwork	0.159
R-free	0.19020
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5ev4
Data reduction software	XDS
Data scaling software	Aimless (0.5.27)
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.210	1.540
High resolution limit [Å]	1.510	1.510
Rmerge	0.050	0.585
Rpim	0.031	0.432
Number of reflections	54400	3555
<I/σ(I)>	14.8	1.6
Completeness [%]	97.7	64
Redundancy	6.4	3.9
CC(1/2)	0.988	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	0.24 M malonate, 26% Peg 3350, 10% sucrose, LDAO