7S3A
Crystal structure of intact U2AF65 RRM-region bound to AdML-C5 oligonucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.245, 63.022, 77.491 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.760 - 1.480 |
R-factor | 0.1324 |
Rwork | 0.131 |
R-free | 0.15730 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5ev4 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.8) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.750 | 1.500 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.042 | 0.196 |
Rmeas | 0.050 | 0.251 |
Rpim | 0.026 | 0.154 |
Number of reflections | 35972 | 1368 |
<I/σ(I)> | 22 | 4.9 |
Completeness [%] | 98.7 | 76.4 |
Redundancy | 6.5 | 3.9 |
CC(1/2) | 0.999 | 0.951 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | 0.60 M succinic acid, 0.10 M Hepes pH 7.0, 2% Peg MME 2000 |