7S0W
Crystal structure of the T337M variant of human PGM-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-12-08 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00001 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 171.693, 171.693, 99.203 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.570 - 2.500 |
R-factor | 0.2173 |
Rwork | 0.214 |
R-free | 0.26960 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5epc |
RMSD bond length | 0.007 |
RMSD bond angle | 0.903 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.570 | 2.580 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.098 | 1.700 |
Rmeas | 0.110 | 1.835 |
Rpim | 0.039 | 0.688 |
Number of reflections | 51770 | 4417 |
<I/σ(I)> | 17.2 | 1.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.7 | 13.8 |
CC(1/2) | 0.999 | 0.747 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 1.8 M ammonium sulfate, 0.1 M MES, pH 6.5, 0.01 cobalt chloride |