7S03
DNA-binding domain of human SETMAR in complex with Hsmar1 terminal inverted repeat (TIR) DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97938 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.978, 166.172, 66.052 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.700 - 2.370 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.23500 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.700 | 2.460 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.044 | 0.459 |
| Rmeas | 0.050 | 0.528 |
| Rpim | 0.024 | 0.256 |
| Number of reflections | 16176 | 1650 |
| <I/σ(I)> | 21.9 | 2.3 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 4.3 | 4 |
| CC(1/2) | 0.999 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M magnesium formate, 15% PEG3350 |
