7RY0
human Hsp90_MC domain structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97914 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.898, 154.260, 156.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.488 - 2.200 |
| R-factor | 0.2071 |
| Rwork | 0.205 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q6m |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.302 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.111 | 0.050 | 1.015 |
| Rmeas | 0.120 | 0.055 | 1.106 |
| Rpim | 0.047 | 0.022 | 0.435 |
| Total number of observations | 317128 | ||
| Number of reflections | 49628 | 5304 | 4888 |
| <I/σ(I)> | 4.8 | ||
| Completeness [%] | 99.9 | 99.7 | 99.7 |
| Redundancy | 6.4 | 6.1 | 5.9 |
| CC(1/2) | 0.999 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Hepes PH 7.5, 20% PEG 400 |
