7RXP

Fab1512 in complex with the C-terminal alpha-TSR domain of P. falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03324
Spacegroup name	P 21 21 21
Unit cell lengths	73.012, 84.149, 90.217
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.397 - 1.761
R-factor	0.1781
Rwork	0.177
R-free	0.20470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Homology model
RMSD bond length	0.011
RMSD bond angle	1.038
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.790
High resolution limit [Å]	1.760	1.760
Rpim	0.033	0.357
Number of reflections	55594	2685
<I/σ(I)>	24
Completeness [%]	99.8
Redundancy	11.5
CC(1/2)	0.960	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	20% PEG3000, 0.2 M sodium chloride, 0.1 M HEPES. pH 7.5