7RX7

Structure of METTL3-METTL14(R298P) mutant methyltransferase complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97938
Spacegroup name	P 41 21 2
Unit cell lengths	101.391, 101.391, 117.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.564 - 1.645
R-factor	0.1677
Rwork	0.167
R-free	0.18770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k7m
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.645	1.645
Rmerge	0.097	0.892
Number of reflections	74404	7285
<I/σ(I)>	23.9
Completeness [%]	99.6
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Tris (pH 8.0) and 20% PEG3350