7RUP

Structure of the human GIGYF2-TNRC6A complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	32.779, 38.319, 62.064
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.425 - 1.230
R-factor	0.1533
Rwork	0.152
R-free	0.17690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fma
RMSD bond length	0.015
RMSD bond angle	1.389
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.430	1.250
High resolution limit [Å]	1.230	1.230
Number of reflections	23420	1130
<I/σ(I)>	13.6	2
Completeness [%]	100.0	100
Redundancy	13	11.7
CC(1/2)	0.999	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.0-1.4 M Na/K phosphate