7RS5
Cryo-EM structure of Kip3 (AMPPNP) bound to Taxol-Stabilized Microtubules
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.9 |
| Number of Particle | 67040 |
| Software | Appion,EMAN2,FREALIGN,CTFFIND,Rosetta |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
