7RQQ

Structure of chimeric antibody F10heavy_L9light with PfCSP peptide NANPNVDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.009
Spacegroup name	P 1 21 1
Unit cell lengths	42.787, 84.944, 64.243
Unit cell angles	90.00, 103.24, 90.00

Refinement procedure

Resolution	42.470 - 1.890
R-factor	0.1638
Rwork	0.161
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rqp
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.470	42.470	1.930
High resolution limit [Å]	1.890	9.060	1.890
Rmerge	0.043	0.024	0.311
Rmeas	0.052	0.029	0.393
Rpim	0.028	0.016	0.236
Total number of observations		1039	3585
Number of reflections	62927	329	1502
<I/σ(I)>	12.8	26.7	2.4
Completeness [%]	93.8	94.8	65.8
Redundancy	3.2	3.2	2.4
CC(1/2)	0.998	0.999	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	0.1M Na Citrate, pH 3.5, 1.5M (NH4)2SO4