7RQP
Structure of PfCSP NPNV binding antibody L9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 67.964, 117.670, 143.665 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.920 - 2.980 |
R-factor | 0.2039 |
Rwork | 0.199 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6d01 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.520 | 45.520 | 3.160 |
High resolution limit [Å] | 2.980 | 8.940 | 2.980 |
Rmerge | 0.092 | 0.036 | 1.050 |
Rmeas | 0.096 | 0.038 | 1.091 |
Rpim | 0.027 | 0.012 | 0.295 |
Total number of observations | 155768 | 5334 | 26112 |
Number of reflections | 12137 | 502 | 1919 |
<I/σ(I)> | 17.5 | 55.3 | 2.6 |
Completeness [%] | 100.0 | 98.9 | 100 |
Redundancy | 12.8 | 10.6 | 13.6 |
CC(1/2) | 0.999 | 0.999 | 0.813 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris, pH 8.0, 100mM NaCl, 8% PEG 20,000 |