7RQE

Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site deacylated tRNA analog CACCA, P-site MAI-tripeptidyl-tRNA analog ACCA-IAM, and chloramphenicol at 2.40A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	209.810, 449.560, 621.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	211.380 - 2.400
R-factor	0.2275
Rwork	0.225
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.004
RMSD bond angle	0.859
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	211.380	211.380	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.246	0.056	2.342
Rmeas	0.261	0.059	2.498
Total number of observations	20837902
Number of reflections	2250029	26249	165237
<I/σ(I)>	7.95	26.81	0.85
Completeness [%]	99.8	99	99.4
Redundancy	9.261	9.457	8.803
CC(1/2)	0.989	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME