7RQC

Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MFI-tripeptidyl-tRNA analog ACCA-IFM at 2.50A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	209.320, 449.630, 620.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	155.050 - 2.500
R-factor	0.2325
Rwork	0.230
R-free	0.27810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.004
RMSD bond angle	0.873
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	155.050	155.050	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.248	0.050	2.409
Rmeas	0.260	0.053	2.517
Total number of observations	23964581
Number of reflections	1986436	23193	145896
<I/σ(I)>	9.53	34.58	1.07
Completeness [%]	99.9	99	99.7
Redundancy	12.064	12.261	11.889
CC(1/2)	0.994	0.998	0.174

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME