7RQA

Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MTI-tripeptidyl-tRNA analog ACCA-ITM at 2.40A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-05
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97895
Spacegroup name	P 21 21 21
Unit cell lengths	209.640, 448.670, 619.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	181.720 - 2.400
R-factor	0.242
Rwork	0.239
R-free	0.28950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.004
RMSD bond angle	0.892
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	181.720	181.720	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.183	0.043	1.474
Rmeas	0.202	0.048	1.646
Total number of observations	12556809
Number of reflections	2234911	25832	163124
<I/σ(I)>	7.05	19.22	0.92
Completeness [%]	99.7	98	98.7
Redundancy	5.618	6.172	5.027
CC(1/2)	0.988	0.996	0.144

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME