7RQ8

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAs, and aminoacylated P-site tRNA at 2.50A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-01
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	209.860, 449.800, 621.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	198.840 - 2.500
R-factor	0.2264
Rwork	0.224
R-free	0.26840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.863
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	198.840	198.840	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.196	0.048	1.974
Rmeas	0.210	0.052	2.114
Total number of observations	15492026
Number of reflections	1995145	23148	146306
<I/σ(I)>	8.58	28.74	1
Completeness [%]	99.8	98.3	99.4
Redundancy	7.765	7.888	7.69
CC(1/2)	0.994	0.997	0.192

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME