7RNV
SH2 domain of guanine nucleotide exchange factor Vav2 in complex with an actin peptide with phosphorylated tyrosine 53
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU MICROMAX-003 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-28 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54184 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 61.492, 109.432, 41.867 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.800 - 2.150 |
R-factor | 0.2339 |
Rwork | 0.230 |
R-free | 0.27190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2iui |
RMSD bond length | 0.002 |
RMSD bond angle | 0.465 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.800 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 7715 | 5024 |
<I/σ(I)> | 10.3 | |
Completeness [%] | 96.1 | |
Redundancy | 13.1 | |
CC(1/2) | 0.987 | 0.312 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 0.2 M sodium acetate trihydrate, 0.1 M Tris hydrochloride, pH 8.5, 30% PEG4000 |