7RM0

Antibody 2E10.E9 in complex with P. vivax CSP peptide ANGAGNQPGANGAGNQPG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-12-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033200
Spacegroup name	P 1
Unit cell lengths	54.520, 66.310, 142.320
Unit cell angles	100.45, 92.31, 91.72

Refinement procedure

Resolution	29.340 - 2.710
R-factor	0.2104
Rwork	0.209
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uyw
RMSD bond length	0.010
RMSD bond angle	1.212
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.340	2.810
High resolution limit [Å]	2.710	2.710
Number of reflections	53126	1111
<I/σ(I)>	7.4	1.6
Completeness [%]	100.0	100
Redundancy	10.4	10.5
CC(1/2)	0.995	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M potassium sodium tartrate and 20% (w/v) PEG 3350