7RKI
Griffithsin-S10Y/S42Y/S88Y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2018-06-01 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.745, 59.655, 67.288 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.910 - 2.090 |
| R-factor | 0.1784 |
| Rwork | 0.173 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2guc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.875 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.17.1) |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.088 | 0.067 |
| Number of reflections | 13650 | 1368 |
| <I/σ(I)> | 8.9 | 1.2 |
| Completeness [%] | 96.3 | 88.6 |
| Redundancy | 7 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M citric acid, pH 3.1, 2.6 M sodium chloride |
