7RKG
Griffithsin mutant Y28W
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU R-AXIS IV |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2019-04-10 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 55.708, 55.708, 183.035 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.160 - 2.200 |
R-factor | 0.1634 |
Rwork | 0.160 |
R-free | 0.19620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2guc |
RMSD bond length | 0.007 |
RMSD bond angle | 0.872 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.17.1-3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.089 | 0.214 |
Rmeas | 0.096 | 0.239 |
Rpim | 0.036 | 0.105 |
Number of reflections | 17186 | 852 |
<I/σ(I)> | 13 | 3.1 |
Completeness [%] | 97.8 | 99.6 |
Redundancy | 6.8 | 3.1 |
CC(1/2) | 0.994 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M citric acid pH 1.5 2.2 M sodium chloride |