7RK7
The complex between TIL 1383i TCR and human Class I MHC HLA-A2 with the bound Tyrosinase(369-377)(N371D) nonameric peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.738, 74.428, 131.058 |
Unit cell angles | 90.00, 93.49, 90.00 |
Refinement procedure
Resolution | 37.210 - 2.540 |
R-factor | 0.2135 |
Rwork | 0.211 |
R-free | 0.25490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qdm |
RMSD bond length | 0.005 |
RMSD bond angle | 0.923 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.310 | 2.631 |
High resolution limit [Å] | 2.540 | 2.540 |
Number of reflections | 36440 | 3527 |
<I/σ(I)> | 8.27 | 2.07 |
Completeness [%] | 99.5 | |
Redundancy | 1.1 | |
CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 16% PEG 3350, 2% tacsimate, 100mM Tris (pH 8.5) |