7RIX

RNA polymerase II elongation complex with hairpin polyamide Py-Im 1, scaffold 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-08-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	167.701, 222.446, 194.157
Unit cell angles	90.00, 100.01, 90.00

Refinement procedure

Resolution	49.530 - 3.400
R-factor	0.2232
Rwork	0.222
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rim
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.530	49.530	3.360
High resolution limit [Å]	3.300	18.070	3.300
Rmerge	0.489
Rmeas	0.525
Rpim	0.189
Number of reflections	105041	638	5177
<I/σ(I)>	3.9
Completeness [%]	100.0	95.3	100
Redundancy	7.7	7.3	7.7
CC(1/2)	0.980	0.999	0.263

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000