7RHY
Cre recombinase mutant (D33A/A36V/R192A) in complex with loxA DNA hairpin
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.91 |
| Number of Particle | 898039 |
| Software | Topaz,cryoSPARC,PHENIX,EPU,ISOLDE |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
