7RHY
Cre recombinase mutant (D33A/A36V/R192A) in complex with loxA DNA hairpin
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.91 |
Number of Particle | 898039 |
Software | Topaz,cryoSPARC,PHENIX,EPU,ISOLDE |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |