7RGN
Crystal structure of putative fructose-1,6-bisphosphate aldolase from Candida auris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 64.477, 64.477, 320.770 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.910 - 2.200 |
R-factor | 0.1983 |
Rwork | 0.194 |
R-free | 0.23830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lnk |
RMSD bond length | 0.003 |
RMSD bond angle | 0.576 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.400 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.098 | 1.440 |
Rpim | 0.033 | 0.493 |
Number of reflections | 21289 | 1032 |
<I/σ(I)> | 21.72 | |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 9.3 | |
CC(1/2) | 0.998 | 0.563 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 10% isopropanol, 0.1 M bicine, pH 8.5, 30% w/v PEG1500, 1 mM zinc chloride |