7RFL
CamA Adenine Methyltransferase Complexed to Cognate Substrate DNA and Inhibitor SGC0946
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.442, 161.741, 229.853 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.870 - 2.380 |
R-factor | 0.2047 |
Rwork | 0.204 |
R-free | 0.23390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7lt5 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.545 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.870 | 2.480 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.206 | 1.780 |
Rpim | 0.062 | 0.615 |
Number of reflections | 111448 | 9690 |
<I/σ(I)> | 10.4 | 1.5 |
Completeness [%] | 91.6 | 80.4 |
Redundancy | 10.8 | |
CC(1/2) | 0.258 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 21~24% (w/v) polyethylene glycol 3350, 0.1 M Tris-HCl pH 7.0~7.5, 0.28 M potassium citrate |