7RFC
Crystal structure of broadly neutralizing antibody mAb1382 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.291, 140.184, 142.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.150 - 3.240 |
R-factor | 0.2358 |
Rwork | 0.233 |
R-free | 0.28420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 6MEH & 6MEI |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 83.670 | 83.670 | 3.400 |
High resolution limit [Å] | 3.240 | 10.750 | 3.240 |
Rmerge | 0.280 | 0.069 | 1.191 |
Rmeas | 0.312 | 0.076 | 1.326 |
Rpim | 0.136 | 0.032 | 0.572 |
Total number of observations | 174067 | 5267 | 22690 |
Number of reflections | 33598 | 1019 | 4415 |
<I/σ(I)> | 4 | 6.7 | 1.5 |
Completeness [%] | 99.1 | 98.1 | 99.2 |
Redundancy | 5.2 | 5.2 | 5.1 |
CC(1/2) | 0.911 | 0.995 | 0.458 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | 0.03 M citric acid, 0.07 M Bis-Tris propane, pH 7.6, 20% PEG3350 |