7RFB
Crystal structure of broadly neutralizing antibody mAb1198 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.090, 102.431, 131.520 |
Unit cell angles | 90.00, 92.73, 90.00 |
Refinement procedure
Resolution | 46.160 - 2.700 |
R-factor | 0.2171 |
Rwork | 0.215 |
R-free | 0.26170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 6MEH & 6MEI |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 80.780 | 80.780 | 2.790 |
High resolution limit [Å] | 2.700 | 10.800 | 2.700 |
Rmerge | 0.093 | 0.082 | 0.888 |
Rmeas | 0.111 | 0.097 | 1.069 |
Rpim | 0.060 | 0.052 | 0.586 |
Total number of observations | 148614 | 2394 | 13869 |
Number of reflections | 47758 | 787 | 4418 |
<I/σ(I)> | 6.4 | 10 | 1.5 |
Completeness [%] | 96.3 | 95.5 | 97.4 |
Redundancy | 3.1 | 3 | 3.1 |
CC(1/2) | 0.991 | 0.989 | 0.610 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 17% PEG10000 |