7REX
PreQ1-1 (type-1) riboswitch in complex with tandem stacked metabolites
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9800 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 57.797, 57.797, 153.635 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.800 - 2.600 |
R-factor | 0.2355 |
Rwork | 0.232 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fu2 |
Data reduction software | XDS (Mar 15, 2019) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.83) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.800 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.096 | 0.880 |
Rpim | 0.071 | 0.677 |
Number of reflections | 17419 | 1382 |
<I/σ(I)> | 8.6 | 2 |
Completeness [%] | 99.6 | 99.1 |
Redundancy | 4.7 | 4.8 |
CC(1/2) | 0.994 | 0.863 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.08 M KCl, 0.012 M NaCl, 30% MPD, 0.04 M NaCacodylate pH 5.5, 0.002 M Hexamine Cobalt (III) Chloride |