3FU2

Cocrystal structure of a class-I preQ1 riboswitch

Summary for 3FU2

Entry DOI	10.2210/pdb3fu2/pdb
Related	3FU4
Descriptor	PreQ1 riboswitch, 7-DEAZA-7-AMINOMETHYL-GUANINE, CALCIUM ION, ... (4 entities in total)
Functional Keywords	rna; aptamer; riboswitch; queuosine; preq1, rna
Total number of polymer chains	3
Total formula weight	33404.81
Authors	Klein, D.J.,Edwards, T.E.,Ferre-D'Amare, A.R. (deposition date: 2009-01-13, release date: 2009-03-03, Last modification date: 2024-02-21)
Primary citation	Klein, D.J.,Edwards, T.E.,Ferre-D'Amare, A.R. Cocrystal structure of a class I preQ1 riboswitch reveals a pseudoknot recognizing an essential hypermodified nucleobase Nat.Struct.Mol.Biol., 16:343-344, 2009 Cited by PubMed Abstract: Riboswitches are mRNA domains that bind metabolites and modulate gene expression in cis. We report cocrystal structures of a remarkably compact riboswitch (34 nucleotides suffice for ligand recognition) from Bacillus subtilis that is selective for the essential nucleobase preQ(1) (7-aminomethyl-7-deazaguanine). The structures reveal a previously unrecognized pseudoknot fold and suggest a conserved gene-regulatory mechanism whereby ligand binding promotes sequestration of an RNA segment that otherwise assembles into a transcriptional antiterminator. PubMed: 19234468 DOI: 10.1038/nsmb.1563 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.85 Å)

Structure validation