7RDH
Crystal structure of the de novo designed binding protein H3mb in complex with the 1968 influenza A virus hemagglutinin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.916, 240.845, 70.704 |
Unit cell angles | 90.00, 117.30, 90.00 |
Refinement procedure
Resolution | 43.240 - 2.750 |
R-factor | 0.235 |
Rwork | 0.232 |
R-free | 0.28890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fnk |
RMSD bond length | 0.002 |
RMSD bond angle | 0.468 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 47866 | 1481 |
<I/σ(I)> | 9 | |
Completeness [%] | 89.2 | |
Redundancy | 3.2 | |
CC(1/2) | 0.790 | 0.310 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.2M sodium thiocyanate and 20% (w/v) polyethylene glycol 3350 as a precipitant |