7RDF

Crystal structure of Pseudomonas aeruginosa D-Arginine Dehydrogenase Y249F co-crystallized in the presence of D-arginine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	60.010, 76.330, 76.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.190 - 1.290
R-factor	0.1278
Rwork	0.126
R-free	0.15650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nye
RMSD bond length	0.005
RMSD bond angle	0.910
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.280	1.360
High resolution limit [Å]	1.290	1.290
Rmerge	0.032	0.150
Number of reflections	86350	11210
<I/σ(I)>	16.35	5.3
Completeness [%]	97.8
Redundancy	7.1
CC(1/2)	0.995	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	13% (w/v) PEG 3350, 6% (w/v) PEG 6000, 20 mM TRIS-Cl, pH 7.0, 10% glycerol, 5 mM D-arginine