7RCM
Crystal Structure of ADP-bound Galactokinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-21 |
Detector | ADSC QUANTUM 1 |
Wavelength(s) | 1.1533 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 128.793, 128.793, 240.652 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.820 - 2.100 |
R-factor | 0.1975 |
Rwork | 0.196 |
R-free | 0.23560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wuu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.680 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.820 | 39.760 | 2.150 |
High resolution limit [Å] | 2.100 | 9.840 | 2.100 |
Rmerge | 0.218 | 0.064 | 1.136 |
Rmeas | 0.223 | 0.065 | 1.254 |
Rpim | 0.046 | 0.011 | 0.515 |
Total number of observations | 25394 | 22383 | |
Number of reflections | 68728 | 810 | 4199 |
<I/σ(I)> | 9.4 | 25.4 | 1.2 |
Completeness [%] | 99.3 | 98.6 | 92 |
Redundancy | 16.7 | 31.4 | 5.3 |
CC(1/2) | 0.997 | 0.999 | 0.568 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 286 | Seeding into drops containing GALK protein at 8 mg/ml, 2.3M Na/K pH6.8 |