7RAJ
Structure of PfCSP peptide 21 with antibody iGL-CIS43.D3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2021-06-23 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.281, 89.712, 72.044 |
| Unit cell angles | 90.00, 99.27, 90.00 |
Refinement procedure
| Resolution | 36.790 - 3.000 |
| R-factor | 0.256 |
| Rwork | 0.253 |
| R-free | 0.31670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6b5l |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.790 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 9207 | 859 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 97.2 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.992 | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.2 M Zn acetate, 0.1 M MES, 17.53% w/v PEG 8000 |
