7RAE
AncAR1 - progesterone - Tif2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 173.15 |
| Detector technology | CCD |
| Collection date | 2013-04-13 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.871, 68.871, 147.281 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.370 - 2.098 |
| R-factor | 0.1939 |
| Rwork | 0.193 |
| R-free | 0.21820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ama |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.584 |
| Data reduction software | PDBSET |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 2.180 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.437 | |
| Number of reflections | 172781 | 21586 |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | HANGING DROP VAPOR DIFFUSION - 20 PERCENT PEG 1000, MES pH 6.5 |
