7R86
Structure of mouse BAI1 (ADGRB1) in complex with mouse Nogo receptor (RTN4R)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.468, 95.315, 69.682 |
Unit cell angles | 90.00, 105.39, 90.00 |
Refinement procedure
Resolution | 38.871 - 1.650 |
R-factor | 0.1656 |
Rwork | 0.165 |
R-free | 0.19450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ozn |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.240 | 40.240 | 1.680 |
High resolution limit [Å] | 1.650 | 9.040 | 1.650 |
Rmerge | 0.081 | 0.025 | 1.544 |
Rmeas | 0.092 | 0.028 | 1.744 |
Rpim | 0.042 | 0.013 | 0.795 |
Total number of observations | 435598 | 2876 | 21390 |
Number of reflections | 94133 | 606 | 4649 |
<I/σ(I)> | 10.7 | 42.2 | 1 |
Completeness [%] | 98.2 | 98.4 | 98.5 |
Redundancy | 4.6 | 4.7 | 4.6 |
CC(1/2) | 0.999 | 0.999 | 0.411 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 1 M succinic acid, pH 7.0, 0.02% w/v 4-Aminobenzioc acid, 0.02% w/v Azelaic acid, 0.02% w/v o-Sulfobenzoic acid monoammonium salt, 0.02% w/v Sodium 4-aminosalicylate dehydrate, 0.002 M HEPES sodium pH 6.8 |