7R7J
Crystal structure of RadD with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-06-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 |
Unit cell lengths | 44.415, 74.449, 109.400 |
Unit cell angles | 84.66, 80.03, 82.77 |
Refinement procedure
Resolution | 35.650 - 2.030 |
R-factor | 0.2373 |
Rwork | 0.236 |
R-free | 0.26940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dje |
RMSD bond length | 0.003 |
RMSD bond angle | 0.608 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (1.19.1_4122) |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.000 | 45.000 | 2.100 |
High resolution limit [Å] | 2.030 | 4.370 | 2.030 |
Rmerge | 0.105 | 0.054 | 1.105 |
Rmeas | 0.124 | 0.064 | 1.378 |
Rpim | 0.066 | 0.034 | 0.810 |
Number of reflections | 79347 | 8647 | 5321 |
<I/σ(I)> | 10.1 | 12.7 | 0.1 |
Completeness [%] | 90.5 | 98.8 | 60.7 |
Redundancy | 3.3 | 3.6 | 2.1 |
CC(1/2) | 0.983 | 0.993 | 0.390 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 277 | 20 mM Tris-HCl, 100 mM potassium chloride, 0.5 mM DTT, 0.5 mM magnesium chloride, 0.5 mM ATPgS, 9% (w/v) PEG 3350, 75 mM sodium thiocyanate |