7R7J
Crystal structure of RadD with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-06-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.415, 74.449, 109.400 |
| Unit cell angles | 84.66, 80.03, 82.77 |
Refinement procedure
| Resolution | 35.650 - 2.030 |
| R-factor | 0.2373 |
| Rwork | 0.236 |
| R-free | 0.26940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6dje |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.608 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (1.19.1_4122) |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.000 | 45.000 | 2.100 |
| High resolution limit [Å] | 2.030 | 4.370 | 2.030 |
| Rmerge | 0.105 | 0.054 | 1.105 |
| Rmeas | 0.124 | 0.064 | 1.378 |
| Rpim | 0.066 | 0.034 | 0.810 |
| Number of reflections | 79347 | 8647 | 5321 |
| <I/σ(I)> | 10.1 | 12.7 | 0.1 |
| Completeness [%] | 90.5 | 98.8 | 60.7 |
| Redundancy | 3.3 | 3.6 | 2.1 |
| CC(1/2) | 0.983 | 0.993 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 277 | 20 mM Tris-HCl, 100 mM potassium chloride, 0.5 mM DTT, 0.5 mM magnesium chloride, 0.5 mM ATPgS, 9% (w/v) PEG 3350, 75 mM sodium thiocyanate |
