7R6R
Crystal Structure of a Mycobacteriophage Cluster A2 Immunity Repressor:DNA Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.11608 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.490, 43.510, 89.280 |
Unit cell angles | 90.00, 102.26, 90.00 |
Refinement procedure
Resolution | 47.390 - 3.130 |
R-factor | 0.2125 |
Rwork | 0.209 |
R-free | 0.24760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7tz1 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.599 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.390 | 3.240 |
High resolution limit [Å] | 3.130 | 3.130 |
Number of reflections | 9005 | 666 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 94.4 | |
Redundancy | 2 | |
CC(1/2) | 0.999 | 0.748 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 5-12% PEG 8,000, 0.1-0.3 M CaCl2, and 0.1M HEPES pH 7.5 |