Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-25 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.92023 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 91.940, 96.990, 119.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.542 - 2.052 |
| Rwork | 0.189 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ows |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.358 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 36379 | 3591 |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.9 | |
| CC(1/2) | 0.998 | 0.871 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 100 mM Tris pH=8.5, 200mM Lithium Sulphate, 20% PEG3350 |
