7R5F
Crystal structure of YTHDF2 with compound YLI_DF_012
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-26 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 65 |
Unit cell lengths | 79.950, 79.950, 113.880 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.980 - 2.000 |
R-factor | 0.2067 |
Rwork | 0.204 |
R-free | 0.24870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rdn |
RMSD bond length | 0.009 |
RMSD bond angle | 0.907 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.980 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 27710 | 4457 |
<I/σ(I)> | 48.15 | |
Completeness [%] | 98.7 | |
Redundancy | 12.3 | |
CC(1/2) | 1.000 | 0.986 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 2.025 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 5.1 |