7R3N

mouse Interleukin-12 subunit beta - p80 homodimer in space group P21 crystal form 3

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	112.813, 65.165, 134.311
Unit cell angles	90.00, 98.09, 90.00

Refinement procedure

Resolution	61.030 - 3.160
R-factor	0.2223
Rwork	0.220
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sff
RMSD bond length	0.010
RMSD bond angle	1.185
Data reduction software	XDS (20210323)
Data scaling software	XDS (20210323)
Phasing software	PHASER (2.8.1)
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	111.670	3.290
High resolution limit [Å]	3.100	3.100
Rmeas	0.160	1.732
Number of reflections	35159	5551
<I/σ(I)>	8.09	0.95
Completeness [%]	99.1	98.2
Redundancy	4.64	4.66
CC(1/2)	0.999	0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	287	BCS screen, 0.2M sodium formate, 0.1M sodium phosphate pH 6.0, 17% PEG Smear Medium, 10% Glycerol. Cryoprotected with up to 27.5% PEG Smear Medium prior to vitrification.