7R3N
mouse Interleukin-12 subunit beta - p80 homodimer in space group P21 crystal form 3
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 112.813, 65.165, 134.311 |
Unit cell angles | 90.00, 98.09, 90.00 |
Refinement procedure
Resolution | 61.030 - 3.160 |
R-factor | 0.2223 |
Rwork | 0.220 |
R-free | 0.25760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sff |
RMSD bond length | 0.010 |
RMSD bond angle | 1.185 |
Data reduction software | XDS (20210323) |
Data scaling software | XDS (20210323) |
Phasing software | PHASER (2.8.1) |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 111.670 | 3.290 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmeas | 0.160 | 1.732 |
Number of reflections | 35159 | 5551 |
<I/σ(I)> | 8.09 | 0.95 |
Completeness [%] | 99.1 | 98.2 |
Redundancy | 4.64 | 4.66 |
CC(1/2) | 0.999 | 0.526 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 287 | BCS screen, 0.2M sodium formate, 0.1M sodium phosphate pH 6.0, 17% PEG Smear Medium, 10% Glycerol. Cryoprotected with up to 27.5% PEG Smear Medium prior to vitrification. |